Intra-Amniotic Autologous PRF & PRP Instillation for Preterm Premature Rupture of Membranes (PPROM)
An innovative, minimally invasive cellular therapy engineered by Dr. Sowmya Dinesh to seal fetal membrane defects, reverse mid-trimester oligohydramnios, and safely prolong high-risk pregnancies to viable term delivery.
Dr. Sowmya Dinesh H R
FRCOG (London) • FRM (Singapore) • MS OBG (RGUHS Rank 2) • Medical Director & Lead InvestigatorThe Clinical Challenge: Second-Trimester PPROM
Preterm Premature Rupture of Membranes (PPROM) before 28 weeks of gestation carries severe fetal morbidity, lethal pulmonary hypoplasia, and sepsis. Conventional obstetrics relies on passive bed rest and antibiotics with poor survival rates. Dr. Sowmya Dinesh’s intra-amniotic PRF/PRP technique provides an active, regenerative therapeutic biological patch that seals the leaky membranes.
When the amniotic sac ruptures prematurely, rapid loss of amniotic fluid (anhydramnios/oligohydramnios) prevents normal fetal pulmonary development and compresses vital umbilical blood flow. By combining concentrated Platelet-Rich Fibrin (which creates a physical gelatinous scaffold) with Platelet-Rich Plasma (which delivers dense vascular endothelial growth factors), cellular adhesion and re-epithelialization of the chorionic membrane are rapidly induced.
4-Step Therapeutic Protocol
Autologous Blood Processing & Fractionation
Under strict Class 10,000 cleanroom protocols, 40ml of maternal venous blood is centrifuged using dual-spin soft centrifugation to isolate platelet-rich fibrin clots and high-concentration growth factor plasma.
Precision High-Resolution Ultrasound Guidance
Using real-time color Doppler sonography, the amniotic defect site or most accessible fluid pocket is mapped to safeguard fetal position and the placental insertion zone.
Amniocentesis-Guided Dual Instillation
A fine 20-gauge spinal needle is guided transabdominally into the amniotic cavity. The PRF scaffold is injected first to form a mechanical biological plug, followed by PRP to stimulate rapid fibroblastic migration.
Serial AFI Monitoring & Tocolytic Support
Amniotic Fluid Index (AFI) is monitored at 24 hours, 72 hours, and weekly intervals alongside antibiotic prophylaxis. Fluid re-accumulation confirms successful sealing.
Verified Clinical Trial Cohort Outcomes
| Parameter | Santasa PRF/PRP Protocol | Conventional Conservative Care |
|---|---|---|
| Membrane Seal Success | 71.4% (10 of 14 cases sealed) | < 15% (Spontaneous seal is rare) |
| Live Birth Rate | 85.7% (12 of 14 pregnancies delivered live) | 30% – 45% in mid-trimester PPROM |
| Pregnancy Prolongation | Up to 149 days (Average +38 days) | Average 4 to 7 days before labor |
| Neonatal Pulmonary Complications | Significantly reduced with normal chest circumference | High incidence of fatal lung hypoplasia |
Experiencing Amniotic Fluid Leaking or PPROM?
Early diagnosis is critical. Contact our emergency fetal medicine desk in Mysuru or Hassan for immediate case evaluation.
Institutional Honors
- 1st IVF & Surrogacy Pioneer: Hassan District (2008).
- Class 10,000 Modular Lab: Ultra-purified cleanroom standards.
- FRCOG London & FRM Singapore: International peer-recognized standards.